Journal of Nanomedicine & Nanotechnology

Gruzman A

Publications

• Research Article
  Gene Silencing by siRNA Nanoparticles Synthesized via Sonochemical Method
  Author(s): Shimanovich U, Munder A, Loureiro A, Azoia NG, Gomes A, Cavaco-Paulo A, Gedanken A and Gruzman AShimanovich U, Munder A, Loureiro A, Azoia NG, Gomes A, Cavaco-Paulo A, Gedanken A and Gruzman A
  
  The knowledge that small RNAs can affect gene expression has had a tremendous impact on basic and applied research, and gene silencing is currently one of the most promising new approaches for disease therapy. However, RNAs cannot easily penetrate cell membranes, therefore RNA delivery become one of the major challenges for gene silencing technology. In the current paper we discuss a general approach for converting siRNA molecules into a dense siRNA nanoparticles using environmentally friendly sonochemical method. The RNA nanoparticulation enhance its gene-silencing activity in vascular bovine endothelial as well as in cancer 293T/GFP-Puro cell lines without causing any toxic effect. We show that ultrasonic waves do not lead to RNA degradation or any changes in its chemical structure. Moreover, sonochemically produced siRNA nanoparticles have been shown to be resistant to a variety of.. View More»
  DOI: 10.4172/2157-7439.1000204

  Abstract PDF