Journal of Aquaculture Research & Development

Giuseppe Scapigliati

Publications

• Research Article
  Analysis of the Expression and Modulation of Selected mmunerelated Gene Transcripts in the DLEC Cell Line from European Sea Bass (Dicentrarchus labrax L.)
  Author(s): Francesco Buonocore *,Elisa Randelli ,Niels Lorenzen ,Katja Einer-Jensen ,Giuseppe Scapigliati Francesco Buonocore *,Elisa Randelli ,Niels Lorenzen ,Katja Einer-Jensen ,Giuseppe Scapigliati
  
  Cell lines have been established from different fish species especially for virus isolation and for studying cell-pathogen interactions, and therefore are of interest in aquaculture. In this paper, we have investigated the presence and the regulation of some immune genes in the DLEC (Dicentrarchus labrax embryonic cells) cell line from European sea bass (Dicentrarchus labrax L.) to preliminary elucidate their action. The basal expression of the selected genes (interleukin- 1? (IL-1?), cyclooxygenase-2 (COX-2), transforming growth factor-? (TGF-?), CD8-?, major histocompatibility complex II-? (MHC II-?), interferon (IFN) and Mx protein (Mx)) have been investigated and, successively, their modulation have been studied both after stimulation with different mitogen agents and after a transfection with a sequence codifying for the coat protein of a fish .. View More»
  DOI: 10.4172/2155-9546.1000105

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