Clinical Microbiology: Open Access

Gisela Pourcel

Publications

• Research Article
  Molecular Genetic Profiling of Clinical and Foodborne Strains of Enterococci with High Level Resistance to Gentamicin and Vancomycin
  Author(s): Gisela Pourcel, Mónica Sparo, Alejandra Corso, Gastón Delpech, Paula Gagetti, María Marta de Luca, Judith Bernstein, Celia Schell, Sabina Lissarrague and Juan Ángel Basualdo Gisela Pourcel, Mónica Sparo, Alejandra Corso, Gastón Delpech, Paula Gagetti, María Marta de Luca, Judith Bernstein, Celia Schell, Sabina Lissarrague and Juan Ángel Basualdo
  
  Enterococci often acquire antimicrobial resistance through horizontal gene transfer. Relatedness between enterococci with high level resistance to gentamicin and vancomycin isolated from humans, food and hospital environment in Tandil County (Argentina) was investigated. PCR amplification for species determination was carried out. Resistance to seven antimicrobials was studied; virulence genes (esp, cylA), vancomycin and gentamicin resistance genes were investigated. In the isolates with high level antimicrobial resistance (gentamicin, vancomycin), pulse-field gel electrophoresis was performed. Vancomycin-resistant E. faecium (n:13) were recovered from human, food and hospital environment samples. All the isolates expressed high-level vancomycin and teicoplanin (vanA genotype), as well high-level gentamicin and streptomycin resistance. Vancomycin-resistant E. faecium were distributed .. View More»
  DOI: 10.4172/2327-5073.1000272

  Abstract PDF