Journal of Microbial & Biochemical Technology

Gauri Bhonde

Publications

• Research Article
  Identification and Characterization of Molecules Having Anti-microbial Activities from Rabbit Epididymis Using Proteomic Approach
  Author(s): Reddy K.V.R, Sukanya Deva, Clara Aranha, Mandar S. Patgaonkar and Gauri BhondeReddy K.V.R, Sukanya Deva, Clara Aranha, Mandar S. Patgaonkar and Gauri Bhonde
  
  Antimicrobial peptides (AMPs) are the key components of the host innate immune system and form the first line of host defense against invading pathogens. In the present study attempts were made to identify and characterize AMPs that are synthesized in the epididymal epithelial cells (EPECs) of rabbit, Oryctologus cuniculus. Ex-vivo induction of EPECs with TLR-9 ligand, CpG-ODN-2006 resulted an up-regulation of several AMPs. These molecules were identified in the acid extracts of pooled epididymal tissue homogenates using proteomic approach (Ultrafiltration, cation-exchange chromatography, RP-HPLC, Far Western blotting - FWB, 2D-PAGE and MALDI-TOF-MS). The pooled RP-HPLC fraction (peaks 1-5), named as rabbit epididymal secretory antimicrobial protein (RESAMP), showed activity against s.. View More»
  DOI: 10.4172/1948-5948.S2-002

  Abstract PDF