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Eugeniusz K Machaj
Eugeniusz K Machaj
Poland
Publications
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Research Article
Isolation and Characteristics of Mesenchymal Stromal Cells from Different Parts of Placenta
Author(s): Ekaterina Semenova, Zbigniew R Mrowiec, Eugeniusz K Machaj, Magdalena Murzyn, Katarzyna Borg, Dariusz Boruczkowski and Tomasz OÅdak Ekaterina Semenova, Zbigniew R Mrowiec, Eugeniusz K Machaj, Magdalena Murzyn, Katarzyna Borg, Dariusz Boruczkowski and Tomasz Ołdak
Placenta performs the following functions: protection, nutrition, respiration, hormone production and excretion. As it is a great source of different cells, we are more and more interested to isolate them from the placenta. Cells can be harvested by non-invasive methods and without any ethical concerns. Due to its structure placenta contains Mesenchymal Stromal Cells (MSCs) of maternal and fetal origin. To make the selection easy, in our experiment we only used placentas from women who gave birth to boys. We used two methods to isolate MSCs (CD - collagenase digestion and MC - mechanical cut) from different parts of the placenta: amnion, chorion, villi and deciduae basalis. MSCs were of CD73+, CD90+, CD105+, CD 14-, CD19-, CD34-, CD45-, HLA-DR- cell surface phenotype, adherent a.. View More»
DOI: 10.4172/2157-7633.1000376