Cardiovascular Pharmacology: Open Access

Erhard Hofer

Publications

• Research Article
  Kif5b Modulates Processing and Secretion of von Willebrand Factor in Endothelial Cells and Mice
  Author(s): Yu-Si Luo, Yue Zhuo, Ke Zhang, Xingde Liu, Erhard Hofer and Jian-Dong Huang Yu-Si Luo, Yue Zhuo, Ke Zhang, Xingde Liu, Erhard Hofer and Jian-Dong Huang
  
  Von Willebrand factor (VWF) secreted by Weibel-Palade bodies within endothelial cells is critical to maintain normal platelet adhesion during vascular arrest. Because the transportation of VWF is microtubule-dependent partly via kinesin activity, suppression of kinesin could affect intracellular trafficking of VWF. In this study, we investigated the role of Kif5b, the key member of the kinesin superfamily, in the processing and secretion of VWF. A hypothetical interaction between VWF and Kif5b was confirmed and the tail domain of Kif5b was identified as VWF binding region. Knocking-down Kif5b in human umbilical vein endothelial cells led to significantly increased non-stimulated VWF secretion, considerably higher ratio of pro-VWF to mature VWF, and shorter VWF length. Consistent to the in vitro assay, Kif5b knockdown mice demonstrated dramatically increased VWF secretion after epineph.. View More»
  DOI: 10.4172/2329-6607.1000249

  Abstract PDF