Journal of Plant Pathology & Microbiology

Eapen SJ

Publications

• Research Article
  Amplification, Cloning and In silico Prediction of Full Length Elicitin Gene from Phytophthora capsici, the Causal Agent of Foot Rot Disease of Black Pepper
  Author(s): Vijesh Kumar IP, Reena N, Anandaraj M, Eapen SJ, Johnson GK and Vinitha KBVijesh Kumar IP, Reena N, Anandaraj M, Eapen SJ, Johnson GK and Vinitha KB
  
  Elicitins are a family of small proteins secreted by Phytophthora, which induce leaf necrosis in infected plants. Here, we report the cloning of Elicitin gene from P. capsici, an Oomycete plant pathogen which causes significant damage to a broad range of host plants. Elicitin sequence was amplified using primers designed from the known Elicitin genes of other Phytophthora organisms based on their conserved motifs. The PCR amplified product size of 256 bp length and the BLAST analysis of the sequenced product showed perfect match with alpha-elicitin sequences of P. capsici. Subsequently, attempt was made to characterize the complete gene of elicitin from genome sequence information of P. capsici, by querying the amplified product against the genome. Local BLAST search against full genome identified entire coding sequence. Further sequence analysis identified promoter sequence, transcri.. View More»
  DOI: 10.4172/2157-7471.1000181

  Abstract PDF