Journal of Plant Pathology & Microbiology

Doostmorad Zafari

Publications

• Research Article
  Characterization of Pseudomonas viridiflava Causing Alfalfa Root Rot Disease in Hamedan Province of Iran
  Author(s): Ali Heydari, Gholam Khodakaramian and Doostmorad ZafariAli Heydari, Gholam Khodakaramian and Doostmorad Zafari
  
  Alfalfa (Medicago sativa) is the most important forage crop in Hamedan province, located in west of Iran. Under field condition, plants showed growth stunting, chlorosis and wilting symptoms not previously reported. To survey the causal agent of the disease, symptomatic plants were collected from the main alfalfa growing area in this province. Plants samples were disinfected, grinded in phosphate buffer, and a loop full of the prepared suspension was stroked on nutrient agar medium containing 5% yeast extract and 1% glycerol. Isolation and characterization of the causal agent, especially from plants in primary growth stage of the disease, is difficult. Isolated bacteria caused wilting and chlorosis after 40 days on alfalfa plants, under green house condition. Phenotypic feature determination of the isolated bacteria, together with two defined strains, by standard bacteriological metho.. View More»
  DOI: 10.4172/2157-7471.1000135

  Abstract PDF