Journal of Plant Pathology & Microbiology

Dinesh Singh

Publications

• Research Article
  Biological Characterization and Genetic Diversity of Indian Strains of Ralstonia solanacearum Biovars 3 and 4 Causing Bacterial Wilt of Tomato
  Author(s): Dinesh Singh, Shweta Sinha, Garima Chaudhary and Yadav DKDinesh Singh, Shweta Sinha, Garima Chaudhary and Yadav DK
  
  Ralstonia solanacearum biovars 3 and 4 causing bacterial wilt of tomato (Solanum lycopersicum L.) is a devastating soil borne plant pathogen across the world. Eighty seven isolates of R. solanacearum were isolated from wilted tomato plants from Jammu and Kashmir, Himachal Pradesh, Uttarakhand, Jharkhand, Orissa states of India and characterized them by traditional and molecular methods. Biovar of R. solanacearum was determined using set of carbon sources and it showed that biovar 3 of R. solanacearum were found most prominent (90.2 per cent) in all the states of India, whereas biovar 4 was found in states of Jharkhand and Himachal Pradesh. The phylotype specific multiplex PCR assigned all 87 the isolates of R. solanacearum infecting tomato under phylotype I. To study the genetic diversity, BOX-PCR and multilocus sequence typing approaches were used. Amplification products yielded in B.. View More»
  DOI: 10.4172/2157-7471.1000443

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