Journal of Nanomedicine & Nanotechnology

Diego Megias

Publications

• Research Article
  Nano-Volume Well Array Chip for Large-Scale Propagation and High-Resolution Analysis of Individual Cancer Stem Cells
  Author(s): Jenifer Clausell-Tormos, Maria M. Azevedo, Irene Miranda-Lorenzo, Catarina R. Vieira, Yolanda Sanchez-Ripoll, Diego Megias and Christopher HeeschenJenifer Clausell-Tormos, Maria M. Azevedo, Irene Miranda-Lorenzo, Catarina R. Vieira, Yolanda Sanchez-Ripoll, Diego Megias and Christopher Heeschen
  
  Cellular heterogeneity represents an increasingly appreciated aspect for research in life science. To address this issue, we have developed a nano-volume well array chip that allows larger-scale isolation and propagation of single cells. Notably, the chip enables single-cell analysis of freshly isolated primary cells at a high-resolution. With an average height of 130 ± 10 μm and an average diameter of 80 ± 10 μm, each nano-volume well can hold up to 0.4 nL of volume, and is compatible with both adherent as well as 3D suspension cultures. Simultaneous time-lapse imaging of thousands of nano-volume wells allows to monitor cell division, as well as tracking of cell fate, and/or alterations in the microscopic cellular morphology and/or markers expression. To demonstrate its application, we employed the system for propagating and tracking of Cancer Stem Cells (CSCs). C.. View More»
  DOI: 10.4172/2157-7439.1000191

  Abstract PDF