Indexed In
- Open J Gate
- Genamics JournalSeek
- Academic Keys
- JournalTOCs
- The Global Impact Factor (GIF)
- China National Knowledge Infrastructure (CNKI)
- Ulrich's Periodicals Directory
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- Google Scholar
Useful Links
Share This Page
Journal Flyer
Open Access Journals
- Agri and Aquaculture
- Biochemistry
- Bioinformatics & Systems Biology
- Business & Management
- Chemistry
- Clinical Sciences
- Engineering
- Food & Nutrition
- General Science
- Genetics & Molecular Biology
- Immunology & Microbiology
- Medical Sciences
- Neuroscience & Psychology
- Nursing & Health Care
- Pharmaceutical Sciences
Déborah Geada
Déborah Geada
Cuba
Publications
-
Research Article
Assessment of a Protein-Free Medium Performance in Different Cell Culture Vessels using Mouse Hybridomas to Produce Monoclonal Antibodies
Author(s): Rodolfo Valdés, Marcos González, Déborah Geada and Eutimio FernándezRodolfo Valdés, Marcos González, Déborah Geada and Eutimio Fernández
The main aim of the study was assessing the performance of a Protein Free Medium (PFM) in different cell culture vessels using three mouse hybridomas to produce monoclonal antibodies (mAb) specific for hepatitis B surface antigen, human alpha interferon and a human versica (proteoglycan). In parallel, the influence of the PFM on the hybridoma production kinetic patterns associated to these vessels was also studied. In conclusions, PFM allowed successful hybridoma cultures and mAb production, but showed limitations compared with serum supplemented medium in T-flask, roller-bottle, gas permeable bioreactors and hollow fiber bioreactors. As it was expected, mAb production kinetic pattern was unmodified by the PFM under assessed experimental conditions and the production kinetic pattern analysis is an important tool for indicating the best operation mode for industrial scale mAb productio.. View More»
DOI: 10.4172/2153-2435.1000155