Biochemistry & Analytical Biochemistry

Colby Cottell

Publications

• Research Article
  Activation of PAK2 by Serum Starvation Sensitizes its Response to Insulin Treatment in Adipocyte 3T3-L1 Cells
  Author(s): Jun Ling, Siska Corneillie, Colby Cottell and Jolinda A Traugh Jun Ling, Siska Corneillie, Colby Cottell and Jolinda A Traugh
  
  p21-activated kinase-2 (PAK2) is ubiquitously expressed in all mammalian cells and tissues tested so far. It is a unique member of PAK family kinases that can be activated by various stress conditions to induce apoptosis or cytostasis. Although many conditions have been reported to activate PAK2, serum starvation followed by insulin treatment has not been studied. In this study, pre-adipocyte (3T3-L1) sensitive to insulin signaling and important for energy homeostasis was used as the system to address this topic. It was found that serum starvation transiently activated PAK2 activity by about 3-fold within one hour, then returning to the basal level within three hours. Following the activation of PAK2 by serum starvation, insulin treatment resulted in a rapid deactivation of PAK2 through ubiquitination-proteasome mediated protein degradation. AKT1 and PAK2 activities were reversely rel.. View More»
  DOI: 10.4172/2161-1009.1000277

  Abstract PDF