Journal of Petroleum & Environmental Biotechnology

Chao Huang

Publications

• Research Article
  Enhanced Rhamnolipid Production of Pseudomonas aeruginosa DN1 by Metabolic Engineering under Diverse Nutritional Factors
  Author(s): Chao Huang, Yanpeng Li, Yuexin Tian, Zhidan Hao, Fulin Chen and Yanling Ma Chao Huang, Yanpeng Li, Yuexin Tian, Zhidan Hao, Fulin Chen and Yanling Ma
  
  Rhamnolipids are a very promising class of biosurfactants exhibiting properties of great interest in several industrial applications, but they are not widely used because of the low yield and the high cost of production. In this study, a metabolic engineering strategy was used to construct the engineered strains DNAB and DNC through introducing rhlAB and rhlC genes respectively to Pseudomonas aeruginosa wild-type strain DN1, as well as optimization of nutritional parameters such as carbon and nitrogen sources were assessed simultaneously, with the purpose of promoting the productivity of rhamnolipids. Both engineered strain DNAB and DNC had higher yield of rhamnolipids than the DN1 under the same conditions by means of increasing the copy number of rhlAB and rhlC genes, respectively. Of particular importance was olive oil and sodium nitrate.. View More»
  

  Abstract PDF