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Cao J
Cao J
China
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Research Article
Vector-Based let-7a miRNAs Function as Suppressors of Multiple Oncogenes in SK-N-MC and SHEP Neuroblastoma Cells
Author(s): Guan J, Guo S, Zeng X, Luo Y, Yang T and Cao J Guan J, Guo S, Zeng X, Luo Y, Yang T and Cao J
Objective: This study was designed to determine the anti-tumor effect of vector-based let-7a miRNA in neuroblastoma cell lines. Methods: A tetracycline (tet)-inducible let-7a expression vector was constructed and used to stably transfect SKN- MC and SHEP neuroblastoma cells. The effects of let-7a overexpression induced by tetracycline on cell proliferation and adhesion were analyzed in vitro by MTT assays and adhesion assays. The mRNA expression of the let-7 target oncogenes NRAS, KRAS, c-myc, and HMGA2 was examined by quantitative real-time reverse transcription polymerase chain reaction. Protein expression of N-RAS, K-RAS, c-Myc, HMGA2, NeuN, and β3- tubulin was analyzed using western blot and immunocytochemistry. Morphology of SK-N-MC and SHEP cells was also observed. Additionally, the let-7a-inducible vector-transfected SHEP cells were subcutaneously injected in nude m.. View More»
DOI: 10.4172/2157-2518.1000248