Journal of Microbial & Biochemical Technology

Brotzmann V

Publications

• Research Article
  Improved Assays to Identify the Antibiotic Effects on Planktonic and Sessile Bacteria Using the Example of 1.8-Cineol
  Author(s): Brotzmann V, Schuermann M, Kaltschmidt B, Kaltschmidt C and Sudhoff HBrotzmann V, Schuermann M, Kaltschmidt B, Kaltschmidt C and Sudhoff H
  
  Screening of antibiotic substances is a mandatory working step during drug development. A variety of methods are available to test their efficiency, they can be divided into diffusion and dilution methods. Diffusion methods in agar based media are rather qualitative approaches, whereas dilution methods, commonly executed in polystyrene microtiter plates, are frequently used to determine the minimal inhibitory concentration (MIC) and the minimal biofilm inhibitory concentration (MBIC50) in a quantitative way. During these standardized assays the physical properties of the agent, e.g. its hydrophobic properties and thermal instability, are often neglected. This study compares different diffusion assays for their sensitivity and improved dilution assays in respect to the thermal sensitivity and the hydrophobic character of antibiotics. We applied 1.8-cineol, a hydrophobic antibacterial c.. View More»
  DOI: 10.4172/1948-5948.1000373

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