Biswadeep Dey
India
Research Article
Inhibition of Fish Pathogenic Aeromonas hydrophila and Edwardsiella tarda by Centella asiatica In-vitro
Author(s): Soumyadip Purkait, Thangapalam Jawahar Abraham, Sutanu Karmakar, Biswadeep Dey and Anwesha Roy
Soumyadip Purkait, Thangapalam Jawahar Abraham, Sutanu Karmakar, Biswadeep Dey and Anwesha Roy
The present study assessed the in-vitro inhibition of fish pathogenic bacteria, viz., Aeromonas hydrophila and Edwardsiella tarda by aqueous, methanol and chloroform extracts of Centella asiatica by agar-disc diffusion, agar overlay well-diffusion, and broth dilution assays. The agar-disc diffusion assay with 10 μL of sterile crude C. asiatica extracts failed to inhibit A. hydrophila; while the crude chloroform extract inhibited E. tarda (11.25 ± 0.35 mm). In agar overlay well-diffusion assay, the methanol and chloroform extracts of C. asiatica(50 μL) inhibited E. tarda at varying levels exhibiting zones of 7.50 ± 0.70 mm and 30.50 ± 6.40 mm, respectively. With the increasing concentration of crude chloroform C. asiatica extract (0-10%/mL), an increased gr.. View More»
DOI:
10.4172/2155-9546.1000524