Bilyana Georgieva
Bulgaria
Research Article
Investigation of Noninvasive Urine Specimens with Molecular Fluctuations for a Presence of High-Risk Human Papilloma Viruses as an Inflammatory Cofactor in the Prostate Cancer
Author(s): Anita Ognyanova Kavrakova, Bilyana Georgieva, Kamen Anachkov, Krassimir Yanev, Georgi Ivanov, Vanyo Mitev and Albena TodorovaAnita Ognyanova Kavrakova, Bilyana Georgieva, Kamen Anachkov, Krassimir Yanev, Georgi Ivanov, Vanyo Mitev and Albena Todorova
Background: The aim of the study is to investigate noninvasive urine specimens in suspected prostate cancer
(PCa) patients by a panel of PCA3, TMPRSS2-ERG fusions and GSTP1 promoter hypermethylation. Furthermore,
we tested urine specimens for the presence of high-risk Human Papilloma Viruses (HPV) as an inflammatory
cofactor in the complicated origin of prostate cancer (PCa).
Methods: A total of 50 patients with elevated PSA and/or PCa physiological symptoms were analyzed. RNA and
DNA isolation; Reverse transcription; Real-time PCR; DNA sequencing; Bisulfite conversion of DNA; Methylationspecific
PCR; Cytological preparations and staining were applied.
Results: Molecular fluctuations were registered in most of the patients: neoplastic GSTP1 allele, PCA3 strongly
elevated expression or hyperexpression. Only in 4 cases a positive TMPRSS2-ERG status was detected. High-risk
HPV type.. View More»
DOI:
10.4172/0974-8369.1000438