Dentistry

Bart De Wever

Publications

• Research Article
  The Evaluation of Sodium Lauryl Sulphate in Toothpaste on Toxicity on Human Gingiva and Mucosa: A 3D in vitro Model
  Author(s): Bart Vande Vannet*,Bart De Wever,Els Adriaens,Frans Ramaeckers,Peter Bottenberg Bart Vande Vannet*,Bart De Wever,Els Adriaens,Frans Ramaeckers,Peter Bottenberg
  
  Five different commercial toothpaste formulations were labeled as A, B, C, D and E and evaluated for toxicity in vitro using reconstituted human oral and gingival mucosa cultures. Toothpastes A, D and E contained sodium lauryl sulphate (SLS) while toothpastes B and C did not. All toothpastes contained fluoride in different quantities. Tissue viability (MTT), tissue morphology (LM and TEM) and release of pro-inflammatory mediator IL-1α were evaluated. For gingival mucosa, topical exposure of toothpastes did not affect MTT values. Examination of oral mucosa however, showed that toothpastes B, E and D induced a significant loss of viability after 1 h (49.2%, 55.5% and 78.4% respectively) (p<0.05). After 3 hours toothpaste B demonstrated a 51.4% loss of viability whereas tissue viability for toothpastes D and E dropped to 11.1% and 4.5% respectiv.. View More»
  DOI: 10.4172/2161-1122.1000325

  Abstract PDF