Journal of Microbial & Biochemical Technology

Baharuddin

Publications

• Research Article
  Comparison of IAA Production by Shallot Rhizosphere Isolated Bacteria in Solid and Liquid Media and Their Effect on Shallot Plant Growth
  Author(s): Kafrawi, Nildayanti, Zahraeni K and BaharuddinKafrawi, Nildayanti, Zahraeni K and Baharuddin
  
  Some of free-living rhizobacteria were isolated from rhizosphereof shallot growing in two different fields on Sulawesi Island. The bacterial isolates were cultured in liquid and solid media and were further tested for their ability to produce bioauxin. L-Tryptophan as a physiological precursor of auxin was added to the culture media and the production of IAA was tested using a colorimeter method. Six isolates from west Sulawesi and ten isolates from South Sulawesi were found to produce bioauxin, while 40 other isolates had negative results on IAA production. The amount of IAA produced by the isolates on liquid medium ranged from 4.01 to 8.62 ppm, while in the solid medium, the concentration of IAA produced by the same bacteria was considerably lower than in Liquid media. Thus, culture conditions influence the secretion of IAA by the bacteria. Of these 16 IAA producing isolates, five e.. View More»
  DOI: 10.4172/1948-5948.1000375

  Abstract PDF