Research Article
Antimicrobial Behavior of Intracellular Proteins from Two Moderately Halophilic Bacteria: Strain J31 of Terribacillus halophilus and Strain M3-
23 of Virgibacillus marismortui
Author(s): Badiaa Essghaier, Cyrine Dhieb, Awatef Rezgui Abdellatif Boudabous, Saida Ayari, Awatef Rezgui and Najla Sadfi-Zouaoui
Badiaa Essghaier, Cyrine Dhieb, Awatef Rezgui Abdellatif Boudabous, Saida Ayari, Awatef Rezgui and Najla Sadfi-Zouaoui
In the present study, we firstly aimed to determine the ability of halophilic bacteria to improve tomato growth. as well as to detect the antimicrobial activities from two moderately halophilic bacteria strain M3-23 of Virgibacillus marismortui and strain J31 of Terribacillus halophilus exhibited by their intracellular proteins. The results showed that both bacteria were able to improve stem tomato growth by comparison of untreated tomato. The halophilic bacteria were also able to produce intracellular antifungal enzyme: glucanase produced by V. marismortui (1.74U/mg) and chitinase (39.39U/mg) produced by T. halophilus. Both chitinases were halotolerants (active in the presence of (0% to 30% NaCl (w/v)). Chitinase produced by strain J31 was alcaline (pH optimum pH 12), but chitinase from strain M3- 23 was acidic (pH 4 optimum) more than 90% .. View More»
DOI:
10.4172/2157-7471.1000214