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Auner AW
Auner AW
Tanzania
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Research Article
Double-Stranded DNA Damage Assessed with Raman Spectroscopy
Author(s): Auner AW and Thomas JC Auner AW and Thomas JC
Double stranded DNA breaks [DSBs] and subsequent repair can correct DNA damage or may mistakenly cause mutations leading to cell damage and disease. DSBs can be by measured with Raman spectroscopy, using inelastic scattered light resulting from distinct molecular vibrations from purified DNA samples. With an exposure time of 20 s and 2 accumulations, Raman analysis found circular pBS KS+ plasmid DNA vibrations were similar to the water blank control. Restriction of the pBS KS+ single EcoR1 site created linear DNA and significant increases in Raman peaks at 880, 1044, 1084, and 1458 cm-1. To further explore Raman detection of DNA damage, human Jurkat lymphocytes were grown in +/- 16 μg/ml of Bleocin™. DNA from the Bleocin™ treated cells demonstrated enhanced Raman absorption at 880, 1044, 1084, and .. View More»
DOI: 10.4172/2161-1009.1000284