Journal of Nanomedicine & Nanotechnology

Ashraf SA El-Saye

Publications

• Research Article
  Immobilization and Characterization of Purified Aspergillus flavus Peroxidase Mediated Silver Nanoparticle Synthesis: Peroxidase Surface Reactive Residues are Implemented for Reduction of Silver Ions, More than Its Active Sites
  Author(s): El-Sayed ASA, Rabie GH, El-Gazzar NS and Ali GSEl-Sayed ASA, Rabie GH, El-Gazzar NS and Ali GS
  
  Silver nanoparticles (AgNPs) have been used extensively in various therapeutic applications, molecular diagnostics. Chemical methods for synthesis of AgNPs are frequently used, however, the expenses and releases of toxic byproducts are the main limitations. Biological methods provide an alternative source for AgNPs synthesis for its cost and environmental safety. Fungi possess unique system for Ag+ resistance via secretion of multiple proteins for ions reduction to their nanoparticles. The objective of this study was to deciphered the mechanisms of Ag+ reduction to Ag0 by A. flavus. The synthesized AgNPs by A. flavus have molecular size ranged from 9-15 nm with visual molecular stability for abundance of stabilizing agents. A. flavus peroxidase was purified, immobilized on chitosan, polyacrylamide and sodium alginate, and biochemically characterized with strong potency for Ag+ reducti.. View More»
  DOI: 10.4172/2157-7439.1000420

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