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Anthony Ananga
Anthony Ananga
Tanzania
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Research Article
Silent Mutation: Characterization of its Potential as a Mechanism for Sterol 14α-Demethylase Resistance in Cercospora beticola Field Isolates from the United States
Author(s): James O Obuya, Anthony Ananga and Gary D. Franc James O Obuya, Anthony Ananga and Gary D. Franc
Sterol demethylation inhibitors (DMIs) are considered among the most effective fungicides used to control Cercospora leaf spot (CLS), caused by Cercospora beticola Sacc., in sugar beet. Resistance to DMI fungicides has been reported in the C. beticola population from the United States, but the molecular mechanism is not known. It is considered that genetic changes in the C. beticola 14α-demethylase (CbCyp51) gene may be contributing to DMI resistance. The study investigated a silent mutation (GAG to GAA) at codon 170 as a potential mechanism for C. beticola DMI resistance. The CbCyp51 gene was obtained from DMI-sensitive and -resistant isolates, cloned into a plasmid vector, transformed in an isogenic yeast R-1, and tested for DMI sensitivity. Transformed yeast showed low .. View More»
DOI: 10.4172/2157-7471.1000280