Journal of Plant Pathology & Microbiology

Alireza Marefat

Publications

• Research Article
  Detection of Pectobacteria Causal Agents of Potato Soft Rot in North Western Provinces of Iran
  Author(s): Sima Azadmanesh, Alireza Marefat and Kayhan AzadmaneshSima Azadmanesh, Alireza Marefat and Kayhan Azadmanesh
  
  Various bacteria belonging to Enterobacteriaceae are known to be agents causing blackleg and soft rot in potato. In this study, a rapid and specific detection method was used to investigate bacteria in some northwestern provinces of Iran (Zanjan, Kordestan and eastern Azarbaijan). 26 strains were selected for the study that had been identified in previous studies as representative pathogens, they were as follows: 14 Pectobactrium carotovorum subsp carotovorum, 7 Dickeya chrysanthemi and 5 Pectobactrium atrosepticum. Primers Y1/Y2, Expccf/r, ADE1/2 and Eca1f/r, published as specific primers for the pathogens. They were tested to determine if they could be used for specific amplification of DNA in the strains. The expected specific fragment was not amplified in many strains or several non-specific bands, some close to those expected fragments were amplified. Specific primers for amplifi.. View More»
  DOI: 10.4172/2157-7471.1000154

  Abstract PDF