Journal of Carcinogenesis & Mutagenesis

Alfredo De Rosa

Publications

• Research Article
  Molecular Profiling of Human Primary Chondrosarcoma-Derived Spheres Reveals Specific and Target Genes Involved in Multidrug Resistance and Metastasis
  Author(s): Vincenzo Desiderio, Francesca Paino, Angela Nebbioso, Lucia Altucci, Giuseppe Pirozzi, Federica Papaccio, Marcella La Noce, Alfredo De Rosa, Gianpaolo Papaccio and Virginia TirinoVincenzo Desiderio, Francesca Paino, Angela Nebbioso, Lucia Altucci, Giuseppe Pirozzi, Federica Papaccio, Marcella La Noce, Alfredo De Rosa, Gianpaolo Papaccio and Virginia Tirino
  
  Chondrosarcoma is a malignant bone tumor that accounts for approximately 25% of all bone neoplasms. The “Cancer Stem Cell” (CSC) hypothesis states that tumor contains a cell subpopulation with stemness features. Sphere cultures are routinely used for self-renewal assays and to select CSCs. Our purpose was to investigate the gene profile of chondrospheres and identify target genes for chondrosarcoma treatment. Whole-genome microarray was used to compare the gene expression of floating spheres with that of adherent counterparts derived from a human primary chondrosarcoma. In addition, CD133, OCT4, SOX2, and collagen type II markers were tested both with Real-Time PCR and flow cytometry, and cell cycle analysis and resistance to cisplatin treatment were performed. Microarray analyses revealed that 1405 genes were found differentially expressed, of which 629 genes were upregul.. View More»
  DOI: 10.4172/2157-2518.1000152

  Abstract PDF