Journal of Microbial & Biochemical Technology

Alain C Roudot

Publications

• Research Article
  Quantifying the Levels of the Mutagenic, Carcinogenic Hydroxylated Aflatoxins (AFM1 and AFM2) in Artisanal Oaxaca-Type Cheeses from the City of Veracruz, Mexico
  Author(s): Estela Hernández-Camarillo, Magda Carvajal-Moreno, Víctor J Robles-Olvera, Manuel A Vargas-Ortiz, Marco A Salgado-Cervantes, Alain C Roudot and Guadalupe Rodríguez-JimenesEstela Hernández-Camarillo, Magda Carvajal-Moreno, Víctor J Robles-Olvera, Manuel A Vargas-Ortiz, Marco A Salgado-Cervantes, Alain C Roudot and Guadalupe Rodríguez-Jimenes
  
  Aflatoxins (AF) are fungal secondary toxic metabolites that have mutagenic and carcinogenic effects in humans. Aflatoxin B1 (AFB1), the most toxic Aflatoxin, contaminates cattle feed and can be metabolized and excreted as the hydroxylate Aflatoxin M1 (AFM1). Aflatoxin B2 (AFB2) is excreted as Aflatoxin M2 (AFM2) in milk, and dairy products such as cheese can concentrate these carcinogens. Artisanal Oaxaca-type cheeses were sampled in Veracruz City, Mexico in 2005 and 2006, and three different extraction methods which were representative of many other reported methods- were selected for testing and validation. The R-Biopharm method was chosen and used to analyze the 30 samples that were derivatized and quantified by HPLC. The validation methods gave limits of detection (LODs) of 0.01 ng g-1 for AFM1 and 0.05 ng g-1 for AFM2; the limits of quantification (LOQs) for each Aflatoxin were f.. View More»
  DOI: 10.4172/1948-5948.1000331

  Abstract PDF