Journal of Aquaculture Research & Development

Ahmed M Hal

Publications

• Research Article
  Phenotypic and Genotypic Characterization of Some Pseudomonas sp. Associated with Burkholderia cepacia Isolated from Various Infected Fishes
  Author(s): Manal I El-Barbary and Ahmed M Hal Manal I El-Barbary and Ahmed M Hal
  
  This study aims to characterize Pseudomonas species that had been isolated from various naturally diseased fresh and marine water fishes, Nile tilapia, catfish, gilt-head bream, and Sea bass using phenotypic method, morphology and biochemistry characters using API 20NE, and genotypic method (16S rRNA gene sequencing) with some of the histopathological characteristics. Six of seven presumptive Pseudomonas sp. were successfully identified by API 20NE method to level species; they were identified as 2 P. fluorescens, one P. putida, one Pseudomonas sp and 3 Burkholderia cepacia while genotypically with 16S rRNA gene sequencing that proved successful for all the four pseudomonas isolates (three as P. fluorescens and one P. putida). The results of phylogenetic analysis placed the isolates in the genus Pseudomonas based on 99% homology. Challenged test revealed that, P. fluorescens and P. pu.. View More»
  DOI: 10.4172/2155-9546.1000499

  Abstract PDF