Journal of Plant Pathology & Microbiology

Achala Bakshi

Publications

• Research Article
  Rapid detection and quantification of Fusarium udum in soil and plant samples using real-time PCR
  Author(s): Sukumar Mesapogu, Bandamaravuri Kishore Babu, Achala Bakshi, Somasani S. Reddy, Sangeeta Saxena, Alok K. Srivastava and Dilip K. AroraSukumar Mesapogu, Bandamaravuri Kishore Babu, Achala Bakshi, Somasani S. Reddy, Sangeeta Saxena, Alok K. Srivastava and Dilip K. Arora
  
  Real-time PCR based detection assay was developed for Fusarium udum, causing vascular wilt of pigeonpea. The Histone-3 gene of F. udum was targeted to design species-specific primers and probe. A comparative study was undertaken to develop a reliable and reproducible procedure for detection and quantification of F. udum from diverse samples. The sensitivity and specificity of oligonucleotides were evaluated through dot blot hybridization, standard and real-time PCR assays. The probe HFUSP showed high degree of sensitivity for DNA obtained from pure cultures of F. udum to that of environmental DNA samples. The qPCR assay specifically differentiated the F. udum from closely related species of Fusarium, other test microbes and environmental samples. The single melting curve at 84.17 and a monomorphic band of 200 bp indicates the specificity and authenticity of the PCR assays. Thus, real-.. View More»
  DOI: 10.4172/2157-7471.1000107

  Abstract PDF