Journal of Vaccines & Vaccination

Abstract

Use of Mesoporous Silica SBa-15 and SBa-16 in Association of Outer Membrane Vesicles - OMV from Neisseria meningitidis

Danilo A Alves, Ives B Mattos, Luciana M Hollanda and Marcelo Lancellotti

Outer membrane vesicles or OMV are nanoparticles released in culture medium during meningococcal growth resulting from evaginations of the outer cellular membrane and have been indicated as potential target for vaccine production. This study aimed to analyze the use of Neisseria meningitidis B2443, as vaccine using a semi-solid fermentation process based in ultrafiltration for the isolation these OMV and also verify the effect of the mesouporous silica (SBA-15 and SBA-16). The OMV preparation follow the method without the ultracentrifugation whose was subtituted by ultrafiltration method using a nitrocelulosis filtre showing a pore of 0.025 μm. For the detection of antibodies production were used the immunological method of ELISA, and serum bactericidal effect using sera from immunized mices with OMV and adjuvant inorganic nanoparticles. Also, the use of citotocity test were performed based in the neutral red uptake for safety of the associated vaccin use in NIH-3T3 cell line. It was compared to OMV production of strains of N. meningitidis strains B2443 and C2135. The results showed that different strains of N. meningitidis have OMVs kinetics of production of different time and quantity. The use of SBA-15 and SBA-16 as adujvant at 250 μg for each mice was enough to induce an increase of vaccinal (for other serogroups) capacity same using a only OMV extracted from strains B2443. The study showed that the methodology used for the production of OMV is advantageous from the point of view of quantity and cost and use of this biologic nanoparticle. Both mesoporous silica SBa15 and SBa16 used in this work were capable to increase de recognition of antibody against different strains fo N. meningitidis showed using the OMV extracted from an only vaccinal strain.