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Abstract
Tissue Dissociation Enzymes for Adipose Stromal Vascular Fraction Cell Isolation: A Review
Ryan A. Lockhart, Cloe S. Hakakian and Joel A. Aronowitz
With the increasing clinical translation of adipose stromal cell based technologies, it is important to thoroughly report on all aspects of these technologies. In this article, we review the commonly used enzymes for adipose tissue dissociation and stromal vascular fraction isolation. The enzymes reviewed include collagenase, trypsin, clostripain, and the neutral proteases dispase and thermolysin. Enzyme characteristics, the advantages of enzymatic isolation, and other relevant practical and regulatory issues are discussed.