Global Journal of Biology, Agriculture & Health Sciences

Abstract

THE SPLICEOSOMAL PROTEIN SnRNP F BINDS TO BOTH U3 AND U14 CLASS OF snoRNA IN Giardia lamblia

Arjun Ghosh, Sumallya Karmakar, Avik K. Mukherjee, Dibyendu Raj, Koushik Das, Srimanti Sarkar, T. Nozaki, & Sandipan Ganguly

Small nuclear Ribonucleo Protein F (snRNP F) is a spliceosomal protein that binds with U1, U2, U4/U6 and U5 small nuclear RNA (snRNA) to form spliceosomal complexes responsible for pre mRNA processing. This study reports the unusual interaction of giardial snRNP F with small nucleolar RNAs (snoRNA) that are responsible for pre rRNA processing. Electrophoretic Mobility Shift Assay was used to demonstrate the interaction of this protein with U3 and U14 class snoRNA of the early branching eukaryote Giardia lamblia. It was also evident from our study that snRNP F in Giardia is evolutionary distinct from its other eukaryotic orthologues.

Published Date: 2013-06-21;