Journal of Tropical Diseases & Public Health

Abstract

The Identification of Microbial Characteristics and Number of Colonies Isolated From River Water in the Region of Mulyoagung Singgahan Tuban

Supiana Dian Nurtjahyani and dan Zainatur Robihah

River is one source of fresh water flowing from upstream to downstream which has a lower surface of the earth’s surface and the flow will lead to sea, lakes, swamps, and others. Nglirip River is one of the Rivers in the village of Mulyoagung Singgahan District of Tuban surrounded by the agricultural sector around the population and it is usually used by people as the fulfilment of daily domestic activities. This study aims to identify the characteristics of microbial colonies were isolated from the River of Nglirip and identify the difference in the number of microbial colonies. The dilution method used was an experimental laboratory. Data were analyzed descriptively and quantitatively with the Mann-Whitney. The result of conducted research using the pour plate method 171 cfu bacterial colonies obtained from the dilution of 10-5 and 116 cfu bacteria colonies of dilution 10-6 and after Mann-Whitney test, there was no significant difference of colonies number of microbes between dilution 10-5 and 10-6 dilutions. The conclusion is that the characteristics of microbial colonies were isolated from River water Nglirip Tuban area Mulyoagung majority are round, white colored milk and belonged to Gram-negative bacteria. And there was no significant difference in the number of colonies of microbes in different dilutions.