Pharmaceutica Analytica Acta

Abstract

Spectrophotometric and Chemometric Methods for Simultaneous Determination of Two Anti-Hypertensive Drugs in their Combined Dosage Form

Khadiga M Kelani, Abdallah A Shalaby, Magda Y Elmaamly and Michael K Halim

In this study determination of Moexipril hydrochloride (MOX) and hydrochlorothiazide (HCT) were conducted by application of Spectrophotometric and Chemometric methods. Five different accurate, sensitive and reproducible methods were applied for the simultaneous determination of (MOX) and (HCT) in their bulk powder and pharmaceutical dosage form. The first method is the new absorbance subtraction (AS) method. The second method is the new amplitude modulation (AM) method. The third method is the new extended ratio subtraction (ERS) method coupled to ratio subtraction (RS) method. The fourth and fifth methods are multivariate calibration which includes Principal Component Regression (PCR) and Partial Least Squares (PLS). The suggested procedures were checked using laboratory prepared mixtures and were successfully applied for the analysis of their pharmaceutical preparations. The validity of the proposed methods was further assessed by applying the standard addition technique. The results obtained by applying the proposed methods were statistically analyzed and compared with a reported method.