Pharmaceutica Analytica Acta

Abstract

Quantitative Determination of Four Angiotensin-II-Receptor Antagonistsin Presence of Hydrochlorothiazide by a Gradient Technique HPLC in their Pharmaceutical Preparations

Hani M. Hafez, Lobna M. Abdelaziz, Abdullah A. Elshanawane and Magda M. Kamal

Losartan potassium, Valsartan , Telmisartan and Irbesartan are angiotensin-II-receptor antagonists (ARA II) group which used in treatment of hypertension alone or in combination with other drugs mainly Hydrochlorothiazide. A gradient HPLC method was developed for the assay of several ARAII in presence of Hydrochlorothiazide. The method was performed by reversed phase high performance liquid chromatography using a mobile phase 0.025 M potassium dihydrogen phosphate (pH 6.0): acetonitrile =80:20% with detection at 220 nm on an ACE C18 column (250 mm × 4.6 mm, 5 μm) at flow rate 1.5 ml/min in a gradient manner. The proposed method was validated in terms of linearity, accuracy, precision and limits of detection and quantitation.