Global Journal of Biology, Agriculture & Health Sciences

Abstract

PRELIMINARY PHYTOCHEMICAL STUDIES AND EFFICACY OF CHLOROFORM EXTRACTS OF CULTURED TISSUES OF PHYSALIS MINIMA (L.) AGAINST PATHOGENS

Harikrishna Ramaprasad Saripalli, Lydia Swapna Nandam, Zenebe Teka3 & Madanprasad

Chloroform extracts of cultured tissues of Physalis minima (L.) family Solanaceae were subjected to preliminary phytochemical analysis and in-vitro antibacterial studies. The solvent fraction revealed the presence of alkaloids, fixed oils, resins, steroids, tannins, xanthoproteins and glycosides. Seven bacterial species Bacillus megaterium (ATCC 23564), Bacillus subtilis (ATCC 6633), Escherichia coli (ATCC 25922), Enterobacter faecalis (ATCC 35550), Proteus vulgaris (ATCC 6380), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), and three fungal species Aspergillus niger (NCIM 596), Aspergillus fumigatus (NCIM 291) and Candida albicans (NCIM 670) were used for the anti-microbial investigations. Chloroform extracts exhibited activity against all tested organisms. The Minimum Inhibitory Concentration (MIC) value was found to be 100µg for E. coli, 250µg for S. aureus, 500µg for B. megaterium, B. subtilis, E. faecalis, P. aeruginosa, P. vulgaris and C. albicans and 750µg for A. niger and A. fumigatus. Among the microbial species tested E. coli was found to be highly sensitive to the chloroform extracts of cultured tissues of Physalis minima (L).

Published Date: 2014-01-02;