Pharmaceutica Analytica Acta

Abstract

Plasmid Mediated Quinolones Resistance ESBL-Enterobactériaceae in Moroccan

EL Malki Fatima, EL Bouraissi Meriem and Barrijal Said

Multidrug Resistance (MDR) in Enterobacteriaceae including resistance to quinolones is rising worldwide. This study was conducted to assess the resistance level to antibiotics and to detect plasmid genes mediated quinolones resistance in Extended-Spectrum β-lactamase (ESBL)-producing Enterobacteriaceae collected from regional hospitals of Fes–Meknes in central Morocco. ESBL phenotype was determined according to the combination disc method recommended by the Clinical and Laboratory Standards Institute (CLSI) using double disc synergy test (DDST). The antimicrobial susceptibility patterns of isolates showed high resistance rate to most antibiotics except imipenem which showed 100% of susceptibility.

A sub-site of 27 isolates was screened for qnr genes by multiplex PCR. qnrB gene was detected in 8 ESBL isolates (2 E. coli, 4 K. pneumoniae, 01 E. aerogenes and 01 C. freundii) while no qnrA neither qnrS could be detected. aac(60)-Ib-cr gene was detected in 15 strains, 13 of them were ESBL.

Our results are in agreement with the general rule that imipenem stays the drug of choice for the treatment of infections caused by ESBL producers. Moreover, the presence of qnr determinants is closely related to ESBL phenotype while aac(60)-Ib-cr gene could be detected in isolates with or without ESBL phenotype.