Pharmaceutica Analytica Acta

Abstract

Mixed Hydrotropy Solubilization Approach for Quantitative Estimation of Eprosartan Mesylate and Hydrochlorthiazide by UV Spectrophotometer

Ruchi Jain, Vinod Sahu, Nilesh Jain and Surendra Jain

Two simple, accurate, novel, safe and precise methods were developed for the simultaneous estimation of poorly water-soluble drugs Eprosartan Mesylate and Hydrochlorthiazide in tablet dosage form using 2M Sodium acetate and 8M Urea solution (50:50% W/V) as a mixed hydrotropic solution. Eprosartan Mesylate and Hydrochlorthiazide show maximum absorbances at 267.5 and 271.5 nm respectively. Sodium acetate and Urea solution did not show any absorbance above 240 nm and thus no interference in the estimation of drugs was seen. Eprosartan Mesylate and Hydrochlorthiazide follows the Beer’s law in the concentration range of 15-75 and 5-25 μg/ml (r 2 = 0.9994 and 0.9996). Method-A employs a simultaneous equation method using 267.5 and 271.5 nm as two analytical wavelengths, Method-B, an absorption ratio method, uses 271.5 and 277 nm as two analytical wavelengths for estimation of Eprosartan Mesylate and Hydrochlorthiazide. The optimized methods showed good reproducibility and recovery with ranging from 95.08±0.086 to 99.82±0.097 EPS and HCZ respectively. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values therefore the both methods can be used for routine monitoring of EPS and HCZ in industry in the assay of bulk drug and tablets.