Biology and Medicine

Abstract

Heterogenous stem cells from nonsorted versus sorted mononuclear cell from bone marrow: FACS based approach

Indumathi S, Dhanasekaran M, Baskaran M, Rajkumar JS,Sudarsanam D

The widely accepted source of stem cell for clinical transplant is the infusion of mononuclear cell (MNC) from bone marrow. The disadvantages of MNC infusion pose a threat to the world of regenerative medicine because of its vast heterogeneity, granulocyte interface and age. Thus, finding an alternative on improvisation of MNC therapy is of utmost importance. Thereby, we hypothesized an alternative approach by sorting of lymphocytes and monocytes using an FACS based purification method. To confirm our speculation, we enumerated various stem cell populations in non-sorted as well as sorted MNC from bone marrow. We found that sorted lymphocytes and monocytes possess high percentage of stem cells when compared to non-sorted MNC. Thus, it is concluded that sorted lymphocytes and monocytes cocktail from bone marrow might be an efficient alternative approach in cell based therapies. However, further investigations on these sorted cells will bring this work closer to clinical applications.