Journal of Biomolecular Research & Therapeutics

Abstract

Gd³⁺-Anionic Linear Globular Dendrimer-G₂-C595 A Dual Novel Nanoprobe for MR Imaging and Therapeutic Agent: An In Vitro Study

Mehdi Mirzaei, Mohammadali Mohagheghi, Daryoush Shahbazi-Gahrouei and Alireza Khatami

Early cancer detection using MR imaging is of high global interest as a powerful non-invasive modality. The aim of this study was to synthesize a nano-dendrimer and its conjugate with C595 mab against breast cancer cell, followed by its chelating with Gd3+. At the end, its ability as a nanosized probe for detection of breast cancer cells was investigated. For this reason, anti-MUC-1 mab C595 was coupled to a biocompatible anionic linear globular dendrimer G2 (ALGDG2) (Poly Ethylene Glycol PEG core and citric acid shell) followed by loading with Gd3+. MR imaging parameters of the prepared nanoconjugate was investigated in vitro performing several studies such as 1H-NMR and 17O-NMR, MUC-1 antigen. The obtained data showed a powerful relaxations as well as selective MUC-1 antigen and cell binding. Findings of this study showed that Gd3+-ALGDG2-C595 nano-probe is potentially, a selective breast molecular imaging agent.