Biology and Medicine

Abstract

Evaluation of antibacterial activity of silver nanoparticles against MSSA and MRSA on isolates from skin infections

Ansari MA, *Khan HM, Khan AA, Malik A, Sultan A, Shahid M, Shujatullah F, Azam A

In recent years, skin and soft-tissue infections (SSTIs), particularly due to multidrug-resistant pathogens are increasingly being encountered in clinical settings. Due to the development of antibiotic resistance and the outbreak of infectious diseases caused by resistant pathogenic bacteria, the pharmaceutical companies and the researchers are now searching for new unconventional antibacterial agents. Recently, in this field nanotechnology represents a modern and innovative approach to develop new formulations based on metallic nanoparticles with antimicrobial properties. The bacterial growth curve, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) of silver nanoparticles (Ag-NPs) towards Staphylococcus aureus ATCC25923, methicillin-sensitive S. aureus (MSSA), and methicillin-resistant S. aureus (MRSA) were examined in this study. The experiment results showed that the lowest MIC and MBC of Ag-NPs to MRSA was 12.5 μg/ml and 25 μg/ml, respectively. The obtained results suggested that Ag-NPs exhibit excellent bacteriostatic and bactericidal effect towards all clinical isolates tested regardless of their drug-resistant mechanisms.