Global Journal of Biology, Agriculture & Health Sciences

Abstract

Establishment of a One-Step Plant Regeneration System in Sweet Potato (Ipomoea batatas [L.] Lam.)

Jin Hee Kim, Kyung-Min Kim & Byung-Wook Yun

We investigated the efficiency of plant regeneration using diverse plant tissues on culture medium containing a combination of plant regulators in sweet potato. Ginhongmi and Sinwhangmi showed the highest callus induction rate among five cultivars of sweet potato tested. The highest shoot and root formation frequencies were acquired from calli derived from internode tissue in the MS medium with 1.0 mg/L NAA and 5.0 mg/L BA. The optimum condition of regeneration media depending on types of tissues was somewhat different, for example, the combination of 0.1 mg/L NAA and 2.0 mg/L BA produced the highest regeneration frequencies for the explants of young leaves. The highest induction of callus, roots and plant regeneration was observed on MS medium with NAA and BA. Taken together, this one-step plant regeneration system skipping hormone shift step can be exploited for the new plant breeding scheme of sweet potato.

Published Date: 2015-02-16;