Journal of Allergy & Therapy

Abstract

Effect of Protein Supplementation on in vitro Maturation of Sheep Oocytes and in vitro Culture of Preimplantation with α-Tocopherol Supplementation in CR1aa Medium on Sheep Embryos to the Blastocyst Stage

Dulam Sreenivas, Kaladhar DSVGK, Nagendra Sastry Yarla, Thomas VM and PalniSamy A

Sheep play a vital role within the Indian economy and therefore the production from native breeds is comparatively low because of their poor generative potency. A great challenge of analysis is concerned to beat the constraints within the technology like costly and sophisticated nature of the technology and low success rate. Fetal bovine serum can be supplemented in IVM and IVF media to achieve better maturation and cleavage. Wheat peptone may not produce desired maturation and cleavage when compared to FBS and BSA, and may not be a useful supplement in IVM and IVF. BSA supplementation also yields better maturation and embryo development when compared to wheat peptone but would involve high cost in embryo production. Supplementation of wheat peptones in IVC of embryos yielded better embryo production suggesting that wheat peptones can be an alternative to animal protein in IVC and would help to minimize the cost in embryo production. The studies have shown that CR1aa medium with α-tocopherol at 200 μM is a better media for in vitro embryo production in sheep.