Journal of Aquaculture Research & Development

Abstract

Effect of Algal Oil Incorporated Diet on Growth Biochemical and Immunological Response in Ornamental Fish Daniorerio rerio

Blessy G, Ajan C, Citarasu T and Michael Babu M

The study was undertaken to know the effect of algal oil as one of the diet ingredients for the betterment of growth, microbial identification and immunological parameters in the fish Daniorerio rerio. The oil was extracted from four micro algae such as Tetraselmis sp., Dunaliella sp., Pavlovasp sp., and Chaetoceros sp., The oil obtained from the four different algae were mixed with other feeding ingredients and fed to Zebra fish Daniorerio rerio. The water quality parameters like temperature, pH, dissolved oxygen, ammonia (NH₃), growth parameters such as weight (absolute growth rate, specific growth rate, food conversion ratio), food consumption and food conversion efficiency were studied biochemically in Daniorerio rerio. Bacterial clearance was evaluated and total viable count of bacteria in different parts of the fish such as gut, gill and body surface were enumerated. Among the oil incorporated diet prepared from the four species of microalgae, Tetraselmis sp., had the maximum growth that ranged from 1.34 g to 2.86 g and control had the minimum growth that ranged from 1.14 g to 2.16 g. The maximum food consumption rate recorded in Pavlova sp., was 0.27 g. Among the total protein estimated in 1^st, 5^th, 10^th, 15^th, 20^th, 25^th and 30^th days, the maximum protein 6.147 mg/ml was noticed in the fishes which are fed with the feed incorporated with oil obtained from Chaetoceros sp. Among the total lipid estimation in 1^st, 5^th, 10^th, 15^th, 20^th, 25^th and 30^th days of feeding, the maximum lipid 6.147 mg/ml was noticed in feed prepared from Dunaliella sp. Among the total carbohydrate estimation in 1^st, 5^th, 10^th, 15^th, 20^th, 25^th and 30^th days, the maximum carbohydrate 2.751 mg/ml was noticed in feed Pavlova sp. Among the total carotenoid estimation in 1^st, 5^th, 10^th, 15^th, 20^th, 25^th and 30^th days of feeding, the maximum carotenoid 0.70 mg/ml was noticed in the animal they are fed with the diet incorporated with oil obtained from Chaetoceros sp. Among the total bacterial clearance noticed in 1, 2, 3 and 4 hours, the maximum bacterial clearance was noticed in feed having Pavlova sp. oil incorporation after 4 hours. It was found that the Zebra fish fed with the diet incorporated with oil from Tetraselmis sp., gave good growth and pigment production.