Journal of Aquaculture Research & Development

Abstract

Effect of 17α, 20β-Dihydroxyprogesterone on In vitro Oocyte Maturation in Persian Sturgeon (Acipenser persicus) and Sterlet (Acipenser ruthenus)

Hajar Azarin *,Mohammad Reza Imanpoor ,Mohammad Pourdehghani

The in vitro effect of 17α, 20β-Dihydroxyprogesterone on the maturation of Persian sturgeon (Acipenser persicus) oocytes for 7, 10, 12, 24 and 30 hr and sterlet (Acipenser ruthenus) oocytes for 12, 18 and 24 hr of incubation was investigated. The oocytes were incubated in SIS, RM2, L-15 and PSACF in the presence or absence of 17α, 20β-Dihydroxyprogesterone at 1 μg/ml concentration. Results showed that in the Persian surgeon, incubation of oocytes after 7, 10, 12, 24 and 30 hr of in hormone free media had no effect on GVBD. The mean oocyte PI in the SIS, L-15, RM2 and PSACF media after 12 hr of incubation was 9.08 ± 4.65, 5.56 ± 3.40, 7.36 ± 2.95 and 5.86 ± 2.54 respectively. During the 24 and 30 hr constant exposure incubation, 17α, 20β-Dihydroxyprogesterone was more effective at inducing GVBD than 7, 10 and 12 of incubation. In the sterlet oocytes, GVBD did not occur after 24 hr of incubation and the mean oocyte PI in the L-15 and RM2 media after 12, 18 and 24 hr of incubation was 10.26 ± 3.63, 5.54 ± 3.59, 6.12 ± 4.86 and 8.6 ± 3.1, 5.98 ± 4.94, 5 ± 4.06 respectively. These results indicated a role for 17α, 20β-Dihydroxyprogesterone on oocyte maturation in the Persian sturgeon.