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Abstract
Development and Validation of RP-HPLC Method for The Simultaneous Estimation of Amoxicillin Trihydrate and Bromhexine Hydrochloride from Oily Suspension
Lalit V. Sonawane and Sanjaykumar B. Bari
A new simple, rapid and precise reverse phase high pressure liquid chromatography (RP-HPLC) method was developed for the simultaneous estimation of amoxicillin trihydrate and bromhexine hydrochloride from oily suspension. An ODS C 18 (250 X 4.5mm ID), 5 μ particle size with mobile phase methanol and glacial acetic acid (50:50 v/v) were used. The flow rate was 1.0ml/min and responses were measured at 254 nm. The retention time for amoxicillin trihydrate and bromhexine hydrochloride were observed at 3.04 and 8.18 min. respectively. Linearity for amoxicillin trihydrate and bromhexine hydrochloride were in the range of 8-50 mcg/ml and 5-25 mcg/mL respectively. Percent recovery was 99.54% and 98.65% for amoxicillin trihydrate and bromhexine hydrochloride respectively. The proposed method can be applied for the routine analysis of amoxicillin trihydrate and bromhexine hydrochloride in combination.