Journal of Food Processing & Technology

Abstract

Detection of invA Gene in Isolated Salmonella from Marketed Poultry Meat by PCR Assay

Indu Sharma and Kashmiri Das

Background: The aims of the current study were to detect the invA gene from chicken samples meant for human consumption from N.E India. Materials and method: After Salmonella sp. identification with culture method, PCR assay were developed for detection of pathogenic genes and antibiotic resistance genes of Salmonella sp. Results: Salmonella was detected in 80 samples of poultry carcasses from main poultry markets in Silchar, Assam, N.E India. A total of 40 Salmonella isolates were found in chicken samples (43%) and the isolates had a growth on brilliant green agar and De-oxycholate citrate agar medium, were oxidase negative and catalase positive and exhibited no changes in the colour of the medium with 100% motility. All the strains were subjected to Salmonella-specific gene (invA) and were confirmed as Salmonella positive by the predicted product of 284-bp DNA fragment. Salmonella isolates recovered from poultry samples were tested for antibiotic susceptibility against 5 selected antibiotics of which ciprofloxacin was observed to be highly susceptible (77.5%). Conclusion: Our results recommended the use of PCR for detection of pathogenic genes of bacteria as a safe, rapid, and accurate method in laboratories. High levels of Salmonellosis infections in poultry farms has raised an eye amongst the poultry management personnel’s to consider various effective control programs to prevent the economic loss resulting from mortality and spreading of infection.