Journal of Clinical and Medical Sciences

Abstract

Detection of Aminoglycoside Resistance Genes in Clinical Isolates of Acinetobacter baumannii

Sumbal Shahzadi*

Acinetobacter baumannii is an emergent nosocomial, gram negative, coccobacilli pathogen that have ability to quickly acquire resistance against multiple antibiotics. Aminoglycoside is class for antibacterial drugs used for pathogenic organism and causes protein synthesis inhibition, irreversible attachment on ribosomal binding site and enzymatic degradation. A. baumannii has acquired resistance against aminoglycosides using multiple genes which produces aminoglycosides modifying enzymes for resistance. The aim of this study was to investigate the prevalence of gene encoding for three different aminoglycoside-modifying enzymes in A. baumannii. 50 strains of A. baumannnii were collected from Mayo Hospital, Services Institute of Medical Sciences and Children Hospital and Institute of Child Health. These strains were characterized by performing biochemical and API test and preceded for antibiotic susceptibility testing. Highest resistance rate was 100% observed for Doxycyclin and Ceftriaxone. Out of 50 drug resistant strains, 38 strains (76%) were found aminoglycosides resistant. After genomic isolation of aminoglycoside resistant strains, PCR was performed to detect aacC1, aadB and aph3 using the specific primers. aacC1 (Acetyletransferases), aadB (“2’-Aminoglycosidenucleotidyltransferase) and aph3 (2’-Aminoglycosidenucleotidyltransferase) were detected with 44%, 34% and 10.5% respectively of drug resistance A. baumannii strains. 24% strains were resistant to aminoglycosides but no resistance gene was detected showing they might involve some other resistance inducing genes. So, there is requirement to further explore other aminoglycoside resistance genes to develop novel drugs.

Published Date: 2025-04-15; Received Date: 2023-10-12