Biology and Medicine

Abstract

Comparative antioxidant activity of different parts of Bauhinia purpurea L.

Kaniz F Urmi, Samina Mostafa, Gulshanara Begum, Tamannatul Ifa, Kaiser Hamid

The present study was undertaken to explore as well as to compare the antioxidant activity of the different plant parts of Bauhinia purpurea L. 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and nitric oxide (NO) scavenging capacity were measured to determine the antioxidant activity of both leaves and bark of the plant. Solvent–solvent partitioning was accomplished to obtain extracts of different polarities as n-hexane, ethyl acetate, and methanol extract. All the extracts exhibited potent antioxidant activity in terms of DPPH and NO scavenging capacity. In case of DPPH radical scavenging, ethyl acetate extract of bark was found to have highest activity with IC50 value of 1.08 µg/mL followed by n-hexane extract of bark and leaves with IC50 values of 2.40 and 3.07 µg/mL, respectively. The IC50 value of standard ascorbic acid was 33.77 µg/mL. In case of NO scavenging activity, the ethyl acetate extract of leaves showed highest activity with IC50 values of 1.04 µg/mL followed by n-hexane and ethyl acetate extract of bark having IC50 values of 1.92 and 2.04 µg/mL respectively. The IC50 value of standard ascorbic acid was 71.06 µg/mL.