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Abstract
Characterization of a new isolate of Bean yellow mosaic virus Group-IV Associated with Mosaic disease of Gladiolus in India
Charanjeet Kaur, Susheel Kumar, Raj SK, Chauhan PS and Neeta Sharma
A new isolate of Bean yellow mosaic virus of group-IV associated with leaf mosaic and flower color breaking symptoms of gladiolus has been characterized based on virus transmission, symptomatology, presence of flexuous rod-shaped particles of 720 nm × 11 nm, Western blot-immunoassay using BYMV antiserum and sequence analysis of full length viral genome (accession number: KM114059, CK-GL2 isolate). The CK-GL2 isolate shared 90-96% nucleotide identity with known BYMV isolates and showed close phylogenetic relationships with phylogenetic group IV isolates of BYMV. Recombination analysis showed eight recombination events in the genome of CK-GL2 isolate and suggested that 80% of the genome was recombinant of six different parental phylogenetic groups of BYMV.