Journal of Biomolecular Research & Therapeutics

Abstract

Callus Induction and Regeneration Capabilities of Indica Rice Cultivars to Salt Stress

Samar SR Sankepally, Venkateswar R Talluri, John P Arulmarianathan and Bharat Singh

The main aim of this study was to test the potentiality of PR-115, PR-116, Sambha Mahsuri, Erramallelu, Cotton Dora Sannalu and Pothana local rice cultivars to NaCl stress. Different concentrations and combinations of growth regulators were supplemented in MS media to achieve the callus induction and regeneration in rice cultivars. Maximum frequency of callus induction (75.54 ± 0.91%) was observed in Sambha mahsuri cultivar with MS+2, 4-D (3 mg/L) supplementation but, the response of 2, 4-D (5.0 mg/L) in callus induction was moderate (70.89 ± 0.45%) in Pothana cultivar of indica rice. The highest frequency of regeneration was achieved (65.45 ± 0.51%) in Sambha mahsuri while lowest in Pothana (42.38 ± 0.73%) cultivar of indica rice at 50 mM NaCl concentration. From the results, it has been established that Sambha Mahsuri was more tolerant to salinity in tissue cultures studies (to 50 mM NaCl concentration) so, this cultivar may be used for better production of rice in saline conditions.